Toni Pfaffeneder

 

Postdoctoral fellow

Location:

HCI G-304

Phone:

+41-44-63-24501

e-mail:

Toni Pfaffeneder

Education

20052010:

Studies of chemistry and biochemistry receiving a bachelor and master degree of science, LMU Munich.

2008–2009:

Research internship with Prof. Birgit Weber, LMU Munich; “synthesis of monomeric and polymeric iron(II) spin crossover compounds and structure-function analysis.”

2009:

Research internship with Prof. Patrick Cramer, LMU Munich, Germany; “crystallization of the subcomplex Med11/22 of the mediator head domain and structure-function analysis.

2009:

Research internship with Prof. Anthony Barrett, Imperial College, London; "total synthesis of angelicoins including a Pd-catalyzed decarboxylative prenylation and aromatization sequence."

2010:

Master thesis with Prof. Thomas Carell, LMU Munich; "synthesis of an unnatural metal-chelating ribonucleoside-5′-triphosphate and initial studies towards its enzymatic incorporation into RNA."

2011–2014:

Ph.D. thesis with Prof. Thomas Carell, LMU Munich; "synthesis and analysis of natural DNA modifications to elucidate the epigenetic DNA metabolism."

2015–present:

Postdoctoral research with Prof. François Diederich, ETH Zurich; “design and synthesis of substituted carbohydrates as inhibitors of tRNA-guanine transglycosylase: replacement of phosphate groups, water Clusters and modulation of enzyme dimerization.“

Design and synthesis of substituted carbohydrates as inhibitors of tRNA-guanine transglycosylase: replacement of phosphate groups, water Clusters and modulation of enzyme dimerization.

Publications

1. T. Pfaffeneder, W. Bauer, B. Weber, Z. Anorg. Allg. Chem. 2010, 636, 183–187. X-ray Structure and Magnetic Properties of Two New Iron(II) 1D Coordination Polymers with Bis(imidazolyle)methane as Bridging Ligand.

2. T. M. Pfaffeneder, S. Thallmair, W. Bauer, B. Weber, New J. Chem. 2011, 35, 691– 700. Complete and incomplete spin transitions in 1D chain iron(II) compounds.

3. W. Bauer, T. Pfaffeneder, K. Achterhold, B. Weber, Eur. J. Inorg. Chem. 2011, 2011, 183–3192. Complete Two-Step Spin-Transition in a 1D Chain Iron(II) Complex with a 110-K Wide Intermediate Plateau.

4. B. Weber, W. Bauer, T. Pfaffeneder, M. M. Dîrtu, A. D. Naik, A. Rotaru, Y. Garcia, Eur. J. Inorg. Chem. 2011, 2011, 3193–3206. Influence of Hydrogen Bonding on the Hysteresis Width in Iron(II) Spin-Crossover Complexes.

5. M. Seizl, L. Lariviere, T. Pfaffeneder, L. Wenzeck, P. Cramer, Nucleic Acids Res. 2011, 39, 6291–6304. Mediator head subcomplex Med11/22 contains a common helix bundle building block with a specific function in transcription initiation complex stabilization.

6. K. Anderson, F. Calo, T. Pfaffeneder, A. J. White, A. G. Barrett, Org. Lett. 2011, 13, 5748–5750. Biomimetic total synthesis of angelicoin A and B via a palladium-catalyzed decarboxylative prenylation-aromatization sequence.

7. J. Cordes, F. Calo, K. Anderson, T. Pfaffeneder, S. Laclef, A. J. White, A. G. Barrett, J. Org. Chem. 2012, 77, 652–657. Total syntheses of angelicoin A, hericenone J, and hericenol A via migratory prenyl- and geranylation-aromatization sequences.

8. T. Pfaffeneder, B. Hackner, M. Truss, M. Münzel, M. Müller, C. A. Deiml, C. Hagemeier, T. Carell, Angew. Chem. Int. Ed. 2011, 50, 7008–7012. The discovery of 5-formylcytosine in embryonic stem cell DNA.

9. M. Münzel, U. Lischke, D. Stathis, T. Pfaffeneder, F. A. Gnerlich, C. A. Deiml, S. C. Koch, K. Karaghiosoff, T. Carell, Chem. Eur. J. 2011, 17, 13782–13788. Improved synthesis and mutagenicity of oligonucleotides containing 5-hydroxymethylcytosine, 5-formylcytosine and 5-carboxylcytosine.

10. T. F. Kraus, D. Globisch, M. Wagner, S. Eigenbrod, D. Widmann, M. Münzel, M. Müller, T. Pfaffeneder, B. Hackner, W. Feiden, U. Schuller, T. Carell, H. A. Kretzschmar, Int. J. Cancer 2012, 131, 1577–1590. Low values of 5-hydroxymethylcytosine (5hmC), the "sixth base," are associated with anaplasia in human brain tumors.

11. S. Schiesser, B. Hackner, T. Pfaffeneder, M. Müller, C. Hagemeier, M. Truss, T. Carell, Angew. Chem. Int. Ed. 2012, 51, 6516–6520. Mechanism and stem-cell activity of 5-carboxycytosine decarboxylation determined by isotope tracing.

12. B. Steigenberger, S. Schiesser, B. Hackner, C. Brandmayr, S. K. Laube, J. Steinbacher, T. Pfaffeneder T. Carell, Org. Lett. 2013, 15, 366–369. Synthesis of 5-hydroxymethyl-, 5-formyl-, and 5-carboxycytidine-triphosphates and their incorporation into oligonucleotides by polymerase chain reaction.

13. C. G. Spruijt, F. Gnerlich, A. H. Smits, T. Pfaffeneder, P.W.T.C. Jansen, C. Bauer, M. Münzel, M. Wagner, M. Müller, F. Khan, H. C. Eberl, A. Mensinga, A. B. Brinkman,    K. Lephikov, U. Müller, J. Walter, R. Boelens, H. van Ingen, H. Leonhardt, T. Carell, M. Vermeulen, Cell 2013, 152, 1146–1159. Dynamic readers for 5-(hydroxy)methylcytosine and its oxidized derivatives.

14. S. Schiesser, T. Pfaffeneder, K. Sadeghian, B. Hackner, B. Steigenberger, J. Steinbacher, G. Kashiwazaki, G. Höfner, K. T. Wanner, C. Ochsenfeld, T. Carell, J. Am. Chem. Soc. 2013, 135, 14593–14599. Deamination, Oxidation and CC bond Cleavage Reactivity of 5-Hydroxymethylcytosine, 5-Formylcytosine and 5-Carboxycytosine.

15. A. S. Schröder, J. Steinbacher, B. Steigenberger, F. A. Gnerlich, S. Schiesser, T. Pfaffeneder, T. Carell, Angew. Chem. Int. Ed. 2013, 53, 315–318. Synthesis of a DNA Promoter Segment Containing all four Epigenetic Nucleosides 5-methyl-, 5-hydroxymethyl-, 5-formyl- and 5-carboxy-dC.

16. D. B. Bucher, B. M. Pilles, T. Pfaffeneder, T. Carell, W. Zinth, ChemPhysChem 2014, 15, 420–423. Fingerprinting DNA oxidation processes: IR characterization of the 5-Methyl-2-deoxycytidine cation.

17. T. Pfaffeneder, F. Spada, M. Wagner, C. Brandmayr, S. Laube, D. Eisen, M. Truss, J. Steinbacher, B. Hackner, O. Kotljarova, D. Schuermann, S. Michalakis, O. Kosmatchev, S. Schiesser, B. Steigenberger, N. Raddaoui, G. Kashiwazaki, U. Müller, C. G. Spruijt, M. Vermeulen, H. Leonhardt, P. Schär, M. Müller, T. Carell, Nat. Chem. Biol. 2014, 10, 574–581. Tet oxidizes thymine to 5-hydroxymethyluracil in mouse embryonic stem cell DNA.

18. M. Wagner, J. Steinbacher, T. F. Kraus, S. Michalakis, B. Hackner,  T. Pfaffeneder, A. Perera, M. Müller, A. Giese, H. A. Kretzschmar, T. Carell, Angew. Chem. Int. Ed. 2015, accepted. Age-dependent levels of 5-methyl-, 5-hydroxymethyl-, and 5-formylcytosine in human and mouse brain tissues.

Authors contributed equally.

Last update: September 2015